18.03.2019
Amoxicillin 400 mg
The double-stranded DNA explained that the essence of life is information and the information is located on these four nucleotides. Every set of three nucleotides on the mRNA forms a codon which codes for a specific amino acid. The four-letter text of nucleotides forms a three-letter codon which codes for an amino acid. There are 64 different combinations of codons which codes for all 20 amino acids. Sequencing human genome identifies the number of nucleotides and the order in which they are arranged. Less than 2% of our genome contains regulatory region, a piece of DNA, which controls the function of genes. More than 300 regulatory regions have been identified. More than 98% of our genome contains non-coding region used to be called the junk DNA which makes up to 60% of our entire genome. The non-coding regions contains repetitive piece of DNA, which is tightly packed and mostly remain silent. The sequencing of this region showed that the non-coding region is the part of viruses and bacteria picked up by our genome during the millions of years of our evolutionary process. During bacterial or viral infection, the non-coding DNA could unfold transcribing into RNA resulting into hazardous protein which could create havoc for our health. As I said above, out of four-letter text, that is A-T and G-C, and three letters code for an amino acid called the codon. The starting codon in a gene is the codon AUG (instead of T nucleotide, we use U nucleotide because thiamin is converted to more water-soluble uracil), which codes for amino acid methionine. The starting codon is followed by a series of hundreds of codons which codes for different amino acids in different species. There are three stop codons, and they are AUG, UGG, and UGA. The longest chain is in a gene of Duchenne muscular dystrophy, a neurological disease whose chain extends to two and a half million codons. Once a gene is identified, using restriction enzymes, like EcoR1, we can cut, paste, and copy all genes individually making a restriction site map. Once a single gene is isolated, we could compare the sequence of this gene with the Thousand Genome Project to identify abnormal mutation responsible for the disease and design drugs to shut off that gene. Let us examine the sequence of the genome of human egg and sperm. An egg contains a single strand of 164 million nucleotide bases carrying 1144 genes, while the human sperm contains a single strand of 59 million nucleotide bases carrying 214 genes. When comparing the sequence of an egg or sperm with sequence of the 1000 eggs and sperms of different people, we notice changes. These variants are mutations caused by radiations, chemical and environmental pollution, viral infection, or genetic inheritance resulting in rare diseases. Once a bad gene is identified, sequencing will identify the abnormal nucleotide. Now, we can design drug to bind to this nucleotide and shut off its function. We present in the “Cancers” section below a novel drug design. Using aziridines and carbamate how we design drugs to shut off these genes to restore health. Although we are allowed to shut off and remove bad genes, we are not allowed to introduce good genes in the egg and sperm to enhance the abilities of egg and sperm because modification introduced in the egg and sperm will pass on to the next 1000 generations. For this reason, germ line gene therapy is forbidden in all countries. Are we to determine the quality of life of individuals who will not even be born before the century is over? We cannot design novel drugs unless we find the abnormal mutations responsible for causing that disease. The reading of the total genetic information that makes us human is called the human genome. The reading of the entire book of our life is authorized by the US Congress under The Human Genome Project. It will answer the most fundamental question we have asked ourselves since the dawn of human civilization. What is the nature of memory and our consciousness? Our development from a single cell to a complete human being? The biochemical nature of our senses and the process of our aging? The scientific basis of our similarity and dissimilarity: similarity is that all living creatures from a tiny blade of grass to the mighty elephant including man, mouse, monkey, mosquitos, and microbes are all made of the same chemical building blocks, yet we are so diverse that no two individuals are alike even identical twins are not exactly identical, they grow up to become two separate individuals. In 1990, US Congress authorized 3 billion dollars to NIH to decipher the entire human genome under the title, “The Human Genome Project.” We found that our genome contains six billion four hundred million nucleotide bases, half comes from our father and another half comes from our mother. Less than 2% of our genome contains genes which code for proteins. The other 98% of our genome contains switches, promoters, terminators, etc. The 46 chromosomes present in each cell of our body are the greatest library of the Human Book of Life on planet Earth. The chromosomes carry genes which are written in nucleotides. Before sequencing (determining the number and the order of the four nucleotides on a chromosome), it is essential to know how many genes are present on each chromosome in our genome. The Human Genome Project has identified not only the number of nucleotides on each chromosome but also the number of genes on each chromosome [4, 5, 6, 7, 8]. The following list provides the details composition of each chromosome including the number of nucleotides and the number of genes on each chromosome. We found that chromosome-1 is the largest chromosome carrying 263 million A, T, G, and C nucleotide bases and has only 2610 genes. Chromosome-2 contains 255 million nucleotide bases and has only 1748 genes. Chromosome-3 contains 214 million nucleotide bases and carries 1381 genes. Chromosome-4 contains 203 million nucleotide bases and carries 1024 genes. Chromosome-5 contains 194 million nucleotide bases and carries 1190 genes. Chromosome-6 contains 183 million nucleotide bases and carries 1394 genes. Chromosome-7 contains 171 million nucleotide bases and carries 1378 genes. Chromosome-8 contains 155 million nucleotide bases and carries 927 genes. Chromosome-9 contains 145 million nucleotide bases and carries 1076 genes. Chromosome-10 contains 144 million nucleotide bases and carries 983 genes. Chromosome-11 contains 144 million nucleotide bases and carries 1692 genes. Chromosome-12 contains 143 million nucleotide bases and carries 1268 genes. Chromosome-13 contains 114 million nucleotide bases and carries 496 genes. Chromosome-14 contains 109 million nucleotide bases and carries 1173 genes. Chromosome-15 contains 106 million nucleotide bases and carries 906 genes. Chromosome-16 contains 98 million nucleotide bases and carries 1032 genes. Chromosome-17 contains 92 million nucleotide bases and carries 1394 genes. Chromosome-18 contains 85 million nucleotide bases and carries 400 genes. Chromosome-19 contains 67 million nucleotide bases and carries 1592 genes. Chromosome-20 contains 72 million nucleotide bases and carries 710 genes. Chromosome-21 contains 50 million nucleotide bases and carries 337 genes.
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